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Journal: Materials Today Bio
Article Title: Stromal homeostasis-restoring “rocket-like” nanomedicine inhibited pancreatic tumor growth in vivo
doi: 10.1016/j.mtbio.2026.103014
Figure Lengend Snippet: In vitro evaluation of Panc-1 and Pan02 cells after different treatments. (A) Colony formation assay of Panc-1 and Pan02 cells after different treatments. (B) Quantification of colony numbers of Panc-1 and Pan02 cells under the indicated treatments. (C) Representative images of cell migration of Panc-1 and Pan02 cells after different treatments. (D) Quantification of residual area of Panc-1 and Pan02 cells in each group. (E) ROS fluorescence intensity of Panc-1 cells after different treatments. (F) ROS fluorescence intensity of Pan02 cells after different treatments. (G) Viability of Panc-1 cells co-cultured with L929 cells in a transwell system after different treatments. (H) Viability of Pan02 cells co-cultured with L929 cells in a transwell system after different treatments. (I) Representative CLSM images of Panc-1 cells co-stained with Calcein-AM (green) and PI (red) after treatment with different groups (scale bar: 200 μm). (J) Representative CLSM images of Pan02 cells co-stained with Calcein-AM (green) and PI (red) after treatment with different groups. (K) Immunofluorescence staining of uPA in Panc-1 cells after different treatments.(scale bar:100 μm). (L) Immunofluorescence staining of uPA in Pan02 cells after different treatments. Data are presented as mean ± standard deviation (SD), n = 3. Statistical significance was analyzed by one-way ANOVA with t -test; ns, not significant; ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: Triethylamine (TEA, Sigma-Aldrich); Cetyltrimethylammonium bromide (CTAB, Xinyanbomei); Sodium salicylate (NaSal, Sigma-Aldrich); Tetraethyl orthosilicate (TEOS, CATO); 1,2-Bis(triethoxysilyl)ethane (BTES, Xinhengyan); Ethanol; Hydrochloric acid; Methanol; Gemcitabine (MedChemExpress); Ammonium bicarbonate (Coolaber); Urokinase-type plasminogen activator (Solarbio); CCK-8 kit (CWBIO); ROS staining kit (Poolyue); Calcein-AM/PI kit (DOJINDO); anti-uPA antibody (HUABIO); Calcium chloride (Supelco); Indocyanine green (zrbiorise); Dulbecco's modified Eagle medium (DMEM, Sigma-Aldrich);
Techniques: In Vitro, Colony Assay, Migration, Fluorescence, Cell Culture, Staining, Immunofluorescence, Standard Deviation